Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria—evolutionary implications
Identifieur interne : 000180 ( France/Analysis ); précédent : 000179; suivant : 000181Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria—evolutionary implications
Auteurs : Jaunius Urbonavic Ius ; Ste Phane Skouloubris [France] ; Hannu Myllykallio [France] ; Henri GrosjeanSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
Formation of 5-methyluridine (ribothymidine) at position 54 of the T-psi loop of tRNA is catalyzed by site-specific tRNA methyltransferases (tRNA:m5U-54 MTase). In all Eukarya and many Gram-negative Bacteria, the methyl donor for this reaction is S-adenosyl-l-methionine (S-AdoMet), while in several Gram-positive Bacteria, the source of carbon is N5, N10-methylenetetrahydrofolate (CH2H4folate). We have identified the gene for Bacillus subtilis tRNA:m5U-54 MTase. The encoded recombinant protein contains tightly bound flavin and is active in Escherichia coli mutant lacking m5U-54 in tRNAs and in vitro using T7 tRNA transcript as substrate. This gene is currently annotated gid in Genome Data Banks and it is here renamed trmFO. TrmFO (Gid) orthologs have also been identified in many other bacterial genomes and comparison of their amino acid sequences reveals that they are phylogenetically distinct from either ThyA or ThyX class of thymidylate synthases, which catalyze folate-dependent formation of deoxyribothymine monophosphate, the universal DNA precursor.
Url:
DOI: 10.1093/nar/gki703
Affiliations:
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In all Eukarya and many Gram-negative Bacteria, the methyl donor for this reaction is S-adenosyl-l-methionine (S-AdoMet), while in several Gram-positive Bacteria, the source of carbon is N5, N10-methylenetetrahydrofolate (CH2H4folate). We have identified the gene for Bacillus subtilis tRNA:m5U-54 MTase. The encoded recombinant protein contains tightly bound flavin and is active in Escherichia coli mutant lacking m5U-54 in tRNAs and in vitro using T7 tRNA transcript as substrate. This gene is currently annotated gid in Genome Data Banks and it is here renamed trmFO. TrmFO (Gid) orthologs have also been identified in many other bacterial genomes and comparison of their amino acid sequences reveals that they are phylogenetically distinct from either ThyA or ThyX class of thymidylate synthases, which catalyze folate-dependent formation of deoxyribothymine monophosphate, the universal DNA precursor.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region></list><tree><noCountry><name sortKey="Grosjean, Henri" sort="Grosjean, Henri" uniqKey="Grosjean H" first="Henri" last="Grosjean">Henri Grosjean</name><name sortKey="Urbonavic Ius, Jaunius" sort="Urbonavic Ius, Jaunius" uniqKey="Urbonavic Ius J" first="Jaunius" last="Urbonavic Ius">Jaunius Urbonavic Ius</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Skouloubris, Ste Phane" sort="Skouloubris, Ste Phane" uniqKey="Skouloubris S" first="Ste Phane" last="Skouloubris">Ste Phane Skouloubris</name></region><name sortKey="Myllykallio, Hannu" sort="Myllykallio, Hannu" uniqKey="Myllykallio H" first="Hannu" last="Myllykallio">Hannu Myllykallio</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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